FastQCFastQC Report
Mon 27 Feb 2023
SRR3126615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170797
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196030.9030323885651216No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43000.19808392954292825No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33610.15482792725436786No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC30110.13870481671017604No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG29410.13548019460133767No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG28390.1307814595284589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67550.083.900131
ACGGGTA2400.078.326634
ATAGCGG4850.073.7009051
TAGGGCA9700.073.643194
AGGGATG37450.072.909655
AGGGAAT27050.072.1010365
AGGGCAT17550.070.962595
AGTAGGG35050.070.7956542
AGGGTAC12000.070.102345
TCCGCTA9800.069.589371
AGAGGGC29850.069.5886843
ACGGGAT6150.069.538774
GTAGGGC9150.069.338333
CTACGAC9800.069.055325
GTAGGGA18350.068.893293
TAGGGTA10050.068.740394
TAGGGAT19500.068.445434
ATAGGGA22800.068.432753
GGATGGC32850.068.3838047
CGTAGGG6500.067.963422
Produced by FastQC (version 0.11.5)