FastQCFastQC Report
Mon 27 Feb 2023
SRR3126614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346131
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101890.7569099887009512No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG41090.30524518044677673No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38740.28778774131195256No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG34450.2559186290190182TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC33150.24626132226358358TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG32080.23831261593411043TruSeq Adapter, Index 15 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15240.11321334996371082No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT14860.11039044491212222No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG14350.10660180918499018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42300.086.092271
AATGCGG2600.079.62381
TACGAGG2350.078.083691
AGGGATG30650.077.892645
CGTAGGG2300.075.633562
CTACGAC7950.075.075775
GGATGGC21050.074.345617
ACGGGAT7850.074.236114
AGGGATT22900.074.085795
GCGTAGG1400.073.9363861
TCCGCTA8100.073.7704241
TAGCGGG6250.073.720242
GAGGGAT29000.073.4113854
AGGGAAT22050.073.318415
TAAGGGA15850.072.940553
AAGGGAT34050.072.8752444
GGAGCTA22250.072.659369
TGTACGG850.071.959471
ATAGCGG1900.071.8138051
CGACGGG2750.071.8054352
Produced by FastQC (version 0.11.5)