FastQCFastQC Report
Mon 27 Feb 2023
SRR3126613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375573
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104950.7629547832067073No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39750.28897048720787627No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG39630.2880981234729091TruSeq Adapter, Index 15 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG38370.2789383042557538No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC37160.27014196992816814TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG36300.26389002982757004TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT16180.11762371026474058No Hit
AAAGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT14700.10686455753347879No Hit
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT14160.1029389207261265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45400.085.6733251
ATAGCGG3750.080.268411
AGGGATG28750.079.765425
TAGCGGG7750.078.214832
AAGGGAT34950.077.313154
CGTAGGG2750.076.891962
ACGGGAT7000.076.531374
CGAAGGG10200.076.473162
TAAGGGA15550.075.551363
AGGGAAT21350.075.4968645
ACGGGTA1250.075.188724
TAAGCGG2850.074.261481
GGATGGC20800.074.104267
GAGGGAT27150.073.9078754
TCCGCTA7550.073.507391
CGCTACG7500.073.3093
GTAGGGA12250.073.270643
GCGTAGG1350.073.161311
AGGGCAT12300.072.9727865
AGGGATT24100.072.536835
Produced by FastQC (version 0.11.5)