FastQCFastQC Report
Mon 27 Feb 2023
SRR3126612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533312
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135700.8850123132147926No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46340.3022215961265548No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG43890.28624311294765836TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC42560.27756907922197177TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG41880.2731342349110944TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT19780.12900179480757995No Hit
AAAGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT16800.10956674179814677No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG16150.1053275523833375No Hit
AGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT15520.101218799565907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51650.086.8513641
CGTAGGG2650.083.356242
ACGGGAT7600.082.866184
ATAGCGG2700.080.11111
AGGGATG32350.079.469255
CGAAGGG11850.077.3396762
TACGAGG2450.076.770171
AAGGGAT38300.076.449634
TAGGGTA4800.076.372934
TAGGGCG2350.075.997944
CTACGAC9050.075.301835
TAAGCGG2000.075.234771
CGGGATT5350.074.6708765
TAAGGGA20200.074.453443
CGCTACG9150.073.965213
AAGGGCA23950.073.3925864
TAGCGGG7900.073.175242
AGGGATC16500.072.9192355
ATAGGGA18950.072.916243
AGGGCGA5350.072.913925
Produced by FastQC (version 0.11.5)