FastQCFastQC Report
Mon 27 Feb 2023
SRR3126611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352316
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273471.1625563912331505No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50800.215957379875833No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC39050.16600660795573383No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG26280.11171968392001755TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG24790.10538550092759645TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89250.085.940651
TAGGGCA11050.075.2825854
ACGGGTA2700.073.1086964
AGTAGGG40450.071.8383942
AGGGATG31100.071.479955
GGTAAGG13900.070.753961
TAAGGGA19550.070.437573
ATAGGGA17400.069.957463
GAGGGAT26350.069.739644
TATAGGG16850.069.484212
TAGGGTA9550.069.390384
ATAGGGC14500.069.363223
ATAAGGG35300.068.9988252
AGGGACT36200.068.5502245
ATGAGGG40050.067.390082
AAGGGGC73600.067.304844
AAGGGAC23100.067.140634
ATAGAGG18050.066.739361
AAGGGTA14100.066.664464
ATAGCGG5250.066.32721
Produced by FastQC (version 0.11.5)