FastQCFastQC Report
Mon 27 Feb 2023
SRR3126610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471939
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278981.1285877200044174No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51290.20748893884517378No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC39330.15910586790369827No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG27700.11205778136110965TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG26600.10760783336482009TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90250.086.073421
ACGGGTA2400.074.401034
ATAGGGA19250.072.2549443
TAGGGCA11050.071.442014
GAATAGG14450.071.309191
AGTAGGG42000.071.2683642
AATAGGG35100.070.1505052
CGTAGGG7650.070.0245062
AGGGATG33500.069.573445
ATAGGGC14650.068.9616243
ACGGGAT5550.068.5801854
AGACGGG17300.068.176432
ATAGCGG5700.067.687461
GGTAAGG13750.067.411311
AAGGGGC76850.067.259784
AGGGACT41150.067.259265
TAAGGGA19450.066.921673
ATAAGGG37150.066.5324552
ATGAGGG40950.066.325512
AGGGAAT21600.065.916715
Produced by FastQC (version 0.11.5)