FastQCFastQC Report
Mon 27 Feb 2023
SRR3126609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808250
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354781.2633490608029911No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58740.20916941155523905No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC42360.15084127125433988No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG30860.10989050120181608TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG29860.10632956467550966TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109500.086.142641
GTAGGGA19550.074.521963
ACGGGTA2450.072.8931664
CGTAGGG7750.072.769412
ACGGGAT7500.072.688564
AGTAGGG45750.072.626922
TAGGGCA14500.072.278014
ATAGGGC17600.072.0975343
AGGGATG37300.071.314355
GAATAGG15200.070.533041
ATAGGGA21850.070.1189653
TAAGGGA25750.069.90223
AAGGGTA18050.068.737874
TAGCGGG18150.068.618082
AGTAAGG19100.068.440431
ATAAGGG40150.068.242092
TAGGGTA10300.067.9858254
TAGAGGG49600.067.179052
GTAGGGC13300.065.7250063
ATAGAGG19550.065.6624761
Produced by FastQC (version 0.11.5)