FastQCFastQC Report
Mon 27 Feb 2023
SRR3126608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557059
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198240.7752656469795964No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42970.16804461688212904No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG27960.10934436788513678No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC26080.10199217147512044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70150.085.267081
GGTAAGG16700.073.8326341
CGTAGGG7900.072.6299442
ATAGCGG5150.072.191051
ACGGGAT7200.071.802134
ATAGGGC15350.070.726273
AGGGAAT26250.070.3623665
ATAGGGA23650.070.148883
AGTAGGG39100.070.005022
TAGGGCA12900.069.950154
GAATAGG16400.068.296211
GTACGGG6400.067.6068652
AGGGATG35550.067.422945
TAGCGGG15500.067.0569152
AGGGCAT20200.066.308725
ACGGGTA2200.066.224124
ATGAGGG45450.066.1226042
CGAAGGG25050.066.087292
GTAGGGC10750.066.015463
AAGAGGG82450.065.997232
Produced by FastQC (version 0.11.5)