FastQCFastQC Report
Mon 27 Feb 2023
SRR3126607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590712
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199340.7694409876512711No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41540.16034202180713256No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG30990.11961962580171011No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC28390.10958377465345435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69400.084.845471
ATAGCGG4750.077.229781
GGTAAGG15550.072.890421
CGTAGGG8350.072.59692
ACGGGAT7350.072.247484
AGTACGG3300.071.2590261
ATAGGGA23950.071.225043
ATAGGGC15850.070.859853
AGGGAAT28000.070.321425
AGTAGGG40250.069.815222
TAGGGAC16750.069.5774464
AGGGATG34750.069.238365
ATAGAGG18200.068.9959641
AGGGCAT20600.067.75185
TAAGGGA19800.067.641193
ATGAGGG42400.066.940042
ATGCGGG11100.066.888112
TAGGGCA12750.066.711394
GTAGGGA19700.066.314753
GAGGGAT29300.065.9182744
Produced by FastQC (version 0.11.5)