FastQCFastQC Report
Mon 27 Feb 2023
SRR3126606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906951
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252940.870121305794284No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48690.16749508333645802No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG33980.1168922351976349No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC31300.10767295355167666No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG29340.10093049384045345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86650.084.2993851
GGTAAGG17550.073.165621
CGTAGGG8000.072.845372
ATAGGGA25350.071.934793
GAATAGG15500.071.614651
GTACGGG8500.071.324882
ATAGGGC18150.070.951113
ACGGGAT9650.070.6197054
AGGGAAT30100.069.795315
TAAGAGG19500.069.225941
AGTAGGG43750.068.320232
TAGAGGG49800.068.2306062
GTAGGGA21600.068.104513
AGTACGG4450.067.64591
GTAAGGG42550.067.2648242
ATAGCGG5750.067.076061
AGGGATG42650.066.999225
TAGCGGG15900.066.800792
TAGGGAC15100.066.607344
AAGAGGG94800.066.232082
Produced by FastQC (version 0.11.5)