FastQCFastQC Report
Mon 27 Feb 2023
SRR3126604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907069
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157380.8252454420894053No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC46890.24587469042808624No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT40120.21037518831253615No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG36970.19385769471372039No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG36840.19317602037472162No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28120.14745140317419034No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27690.14519663420673296No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20840.10927764019026055No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT19690.10324744411450242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55500.083.8023151
AGGGATG34450.079.667315
CGTAGGG6200.077.311172
AGGGCAT16650.075.079955
ACGGGAT6300.073.850124
TAGGGCG3800.072.966744
ATAGGGA18050.072.901663
ACGGGTA2200.072.6294564
TAGGGCA10650.072.36874
TAGGGTA5600.071.33254
AGGGTAC7850.071.241635
GGTAAGG10650.071.0933461
TAGGGAT16250.070.56564
CTAACGG2000.070.541381
AAGGGTA11350.070.389784
ATAAGGG24550.070.250332
GTAGGGA16850.069.726313
TAGGGAC11400.069.668814
AGTAGGG30550.069.52822
GTAGGGC9400.069.493393