FastQCFastQC Report
Mon 27 Feb 2023
SRR3126603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114841
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196560.9294315742885636No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC51000.24115288099672744No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG43010.20337226297390676No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG40490.19145647355995085No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34120.16133600587467334No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31580.14932564670346377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69050.083.16681
CGTAGGG6300.082.061982
TAGGGTA7850.082.023974
AGGGATG40750.078.1973345
AGGGTAC8700.077.251465
AGGGCAT19400.076.3130345
AGGGTAG10150.075.0134055
ACGGGAT7200.074.4153064
GTAGGGA20250.074.270243
ATAGGGA19200.073.680943
AGTAGGG38300.073.259752
AAGGGTA14500.073.253824
GGTAAGG11250.073.161671
TAGGGAC12550.072.2775654
TAGGGCA12600.071.990744
ATAGAGG15600.071.754711
TAGGGAT18100.071.667194
GTAGGGC9850.071.095143
GAATAGG14100.071.049091
TAAGGGA20200.070.964043
Produced by FastQC (version 0.11.5)