FastQCFastQC Report
Mon 27 Feb 2023
SRR3126602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645360
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139930.8504521806777848No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32010.19454708999854137No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC20350.12368113968979433No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19890.1208853989400496No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC19410.1179681042446638No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG18460.1121942918267127No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG18300.11122186026158411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48450.084.402431
ATAGCGG3850.079.447751
CGTAGGG4450.076.075492
AGGGATG30950.074.859575
ACGGGAT4500.074.149284
TAGGGCA9300.073.273364
TACGACC6400.072.696976
CTACGAC6500.071.578555
AGGGCAT16600.071.343355
GTAGGGC7650.070.033283
GAGGGAT24100.070.0065164
GGATGGC26300.069.5112157
ATAGGGA16300.069.484913
ATGGCAC24200.069.328849
ATAGGGC10850.069.302883
GAATAGG10600.069.254451
CGAAGGG19200.069.058982
AGTAGGG27050.068.659742
AGGGTAC7900.068.411935
AAGGGTA11200.068.3959964
Produced by FastQC (version 0.11.5)