FastQCFastQC Report
Mon 27 Feb 2023
SRR3126601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684033
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141490.8401854357960918No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33850.20100556224254512No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG20890.12404745037656625No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG20520.12185034378779988No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC20480.1216128187511765No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18880.11211181728624084No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC18690.11098357336227972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48700.084.9847261
AGGGATG31800.076.697135
ATAGCGG3350.075.8116761
GTACGGG3500.075.187562
ATAGGGC10000.074.2499163
CGTAGGG4750.073.208932
ACGGGAT4250.072.978324
GACCGAT650.072.300028
GAATAGG9600.071.036891
GTAGGGC7050.070.657073
AGGGTAC9250.070.617435
CTACGAC7850.070.041445
TAGGGCA9550.069.875334
AGTAGGG25050.069.784862
GAGGGAT24450.069.769664
AGGGAAT20850.069.645225
TAGGGTA5400.069.620174
TATAGGG13400.069.085582
GGATGGC26650.068.7731867
TAGGGCG2050.068.771154
Produced by FastQC (version 0.11.5)