FastQCFastQC Report
Mon 27 Feb 2023
SRR3126600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899393
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181740.9568319984331837No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38990.20527610662985488No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG22480.11835360033442263No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG22470.11830095193569735No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22320.11751122595481821No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC22250.11714268716374125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62050.084.146931
ATAGCGG4750.077.242811
AGGGATG32750.072.613745
TAGGGCA9150.072.423194
CTACGAC9700.071.2238165
ATAGGGC12450.070.968913
TAGAGGG36400.070.109692
ATAGGGA17450.070.025713
AGTAAGG12250.069.886351
AATGCGG3400.069.174841
GTAGGGT9000.068.930443
TAGCGGG12550.068.905472
ATAAGGG27300.068.861582
GTAGGGA17750.068.312623
AGATAGG11450.068.196111
TAGGGAT15550.068.003574
TAAGGGA17450.067.332413
TACGACC10200.067.269916
AGGGTAC8350.066.979225
AAGGGCA30850.066.8788764
Produced by FastQC (version 0.11.5)