FastQCFastQC Report
Mon 27 Feb 2023
SRR3126599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943966
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177720.914213520195312No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37550.19316181455848508No Hit
AAGAGAGGTGCATGTTGGTATTTAAAGTAGTGGATTCAAAGAACTTAGAT19520.10041327883306601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59050.083.3601461
GAATAGG10550.073.600071
CGTAGGG5500.073.511892
AGGGATG28000.071.66785
ACGGGAT5350.069.39494
ATAGCGG4350.069.236871
ATAGGGA16500.068.6415943
AGTAGGG32850.067.550482
GGTAAGG12300.067.3370741
TAGCGGG12550.067.0552
AGACGGG13150.066.855582
ATAAGGG28650.066.786952
TAAGGGA18000.066.5766453
GTAGGGA15200.066.1644063
TAGGGCA8500.065.7933964
TAGGGAT13650.065.7589654
GAGGGAT22650.065.357674
ATAGAGG14050.065.3138051
AGGGACT28700.065.171165
TGTAGGG15550.065.002482
Produced by FastQC (version 0.11.5)