FastQCFastQC Report
Mon 27 Feb 2023
SRR3126598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980627
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178460.9010278058412816No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35810.18080133210341978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60350.083.6980061
CGTAGGG6600.075.472382
TAGGGCA9500.074.198274
ATAGGGC10150.073.615323
AGGGATG27300.069.369495
GAATAGG11300.068.6741941
GTAGGGC9850.068.223953
AGTAGGG33050.067.964572
AGTAAGG12100.066.854581
AGGGACT30350.066.733625
ATAGAGG15250.066.306591
ATAGGGA15900.066.2045753
GTAGGGA14700.066.1744163
TAAGGGA17250.065.9273
AAGGGGC62600.065.30874
AATAGGG27400.065.171722
AAGGGAC19400.064.917114
ACGGGAT5300.064.725164
GTACGGG5300.064.725162
GGTAAGG12950.064.64551
Produced by FastQC (version 0.11.5)