FastQCFastQC Report
Mon 27 Feb 2023
SRR3126597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225720
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224891.0104146074079399No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41740.18753482019301618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73800.085.051481
GAATAGG13800.072.283511
CGTAGGG7550.070.96952
ATAGGGA18900.069.6321953
AGGGATG33850.069.565345
TAGGGCA10950.069.5368044
TAAGGGA19100.068.903063
TAGAGGG41500.068.407342
AGGGAAT21350.067.5856255
AGTAGGG37500.066.9304662
ATAAGGG31650.066.084582
ATAGGGC12150.065.763743
GTACGGG6650.065.731742
GAGGGAT25800.065.219454
GTAGGGA16100.065.101773
AGACGGG13800.065.053122
AAGGGAT36800.064.75514
ATAGAGG17150.064.748591
AGGGCAT17750.064.6108255
AGGGATA21050.064.529695
Produced by FastQC (version 0.11.5)