FastQCFastQC Report
Mon 27 Feb 2023
SRR3126594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194566
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210900.9610100584808111No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48550.22122825196416968No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG46130.2102010146880978RNA PCR Primer, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG40510.18459230663374898RNA PCR Primer, Index 38 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC34760.1583912263290327RNA PCR Primer, Index 38 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25760.11738083976512896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67350.084.442011
AGGGATG32450.075.458265
GTACGGG3750.073.944262
AGGGCAT18850.073.551995
ACGGGTA2050.073.363474
AGTACGG2650.072.77931
AAGGGAC21750.072.388454
TAGGGCA13900.072.357364
ACGGGAT6850.071.355354
TAAGGGA19450.071.041393
AGGGAAT27800.070.159595
TAGCGGG12600.070.124712
AGTAGGG37700.070.0613862
TAGGGCG3500.069.82634
ATGCGGG11250.069.348862
ATAGCGG4700.069.059131
AAGGGTA14900.068.762874
TAGAGGG37100.068.660842
TAGGGTA8850.068.506254
AAGAGGG67600.068.411982
Produced by FastQC (version 0.11.5)