FastQCFastQC Report
Mon 27 Feb 2023
SRR3126593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979013
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204281.0322317235915075No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33850.17104485923033352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71250.085.807071
ATAGCGG4950.072.261531
GAATAGG11750.070.0969241
ATAGGGC12000.068.929373
TAAGGGA18950.068.201883
TAGGGCA10950.067.813454
ACGGGTA2500.067.67614
TATAGGG15600.067.5327762
ATAGGGA16300.067.468513
CGTAGGG6000.066.628322
AGGGTAC10400.066.428875
AGGGATG27100.066.420545
AGTAGGG35800.065.949562
ACGGGAT5850.065.876564
AGGGCAT16100.065.679455
AAGAGGG70450.065.490642
GGTAAGG11900.065.25831
GTAGGGA15800.065.141823
GTACGGG4850.064.97172
TAGAGGG38050.064.6454852
Produced by FastQC (version 0.11.5)