FastQCFastQC Report
Mon 27 Feb 2023
SRR3126592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046255
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206011.006766018897938No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35200.17202157111405958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70800.086.636791
ATAGGGC11650.072.2003253
ATAGGGA17500.071.425893
TAGGGTA6550.071.0241244
GAATAGG12350.070.082381
AGGGATG29100.069.7594155
GTAGGGA15650.068.459313
TAAGGGA15700.068.2412953
TAGGGCA10500.067.5771264
AGATAGG12600.066.8252261
TAGGGAC12250.066.7459954
GATAGGG30150.066.706562
ATAGCGG6700.066.6970751
AGTAGGG38050.066.688532
GAGGGAT24200.066.408364
TAGAGGG40400.066.1824652
ATAGAGG15000.066.1681751
ACTAGGG10250.066.016222
AGGGTAC10950.065.658265
AGGGACT28700.065.492295
Produced by FastQC (version 0.11.5)