FastQCFastQC Report
Mon 27 Feb 2023
SRR3126591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288229
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252771.104653424110961No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39590.17301590006944234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85000.085.612291
ATAGCGG6650.075.6879651
TAGGGCA12100.071.4673844
ACGGGAT5950.069.509024
ATAGGGC12750.069.298383
GTAGGGA17850.068.719153
ATAGGGA19300.068.670033
AGGGATG31400.068.1015855
TAAGGGA18850.068.065453
GAATAGG12400.067.9041061
AGTAAGG15050.067.824711
AGGGAAT24750.067.410665
AGTAGGG37650.066.907572
TAGAGGG43350.066.024272
GTAGGGC10700.065.445213
TAGCGGG15400.065.308322
TAGGGAT15400.065.308324
ATAAGGG32650.064.7746052
AAGAGGG78100.064.689372
GATAGGG36050.064.662412
Produced by FastQC (version 0.11.5)