FastQCFastQC Report
Mon 27 Feb 2023
SRR3126590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897865
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165050.869661435349722No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44240.2331040405929821No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG23360.12308567785379887No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC20430.10764727733532155No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG19200.10116631056476619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56850.082.61191
ACGGGTC2350.075.997154
CGTAGGG6150.075.703572
ATAGGGA15850.072.054083
TAAGGGA14750.072.010863
AGTAGGG32700.071.76442
AGATAGG10450.071.601711
TAGGGCA9550.070.866454
TAAGCGG3950.070.2906341
AGGGATG24100.070.009825
GCATAGG4450.069.795351
GTACGGG4650.069.783442
GTAGGGT8200.068.77793
GTAGGGC9100.067.65683
ATAGGGC9050.067.511283
TAGGGTA5500.067.506554
AGTAAGG9400.067.084081
ACGGGAT5750.067.023574
TGTAGGG13200.066.9792252
ATAAGGG25300.066.731392
Produced by FastQC (version 0.11.5)