FastQCFastQC Report
Mon 27 Feb 2023
SRR3126589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012890
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168310.8361609427241429No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45430.22569539319088472No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG26380.13105534828033324No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG21090.10477472688522473No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC21050.10457600763081937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54400.084.394171
CGTAGGG6500.077.353262
TAGGGTA5550.073.658644
GGTAAGG10050.072.548291
AGGGAAT19000.072.214885
TAGAAGG17250.070.627241
AGGGATG25450.070.5298545
AGTAGGG33250.070.5207752
GTAGGGC9500.069.743493
ACGGGAT5400.069.613534
TAAGGGA14550.069.4358443
TAGGGCA9100.069.192794
TAACGGG7150.069.006732
ATAGGGA15700.068.240693
GAATAGG13350.068.004491
ATAGGGC10150.067.591963
TAAGCGG4650.066.7655641
AGTAAGG13050.066.684171
AATAGGG27450.066.590992
TAGGGAT11550.066.313674
Produced by FastQC (version 0.11.5)