FastQCFastQC Report
Mon 27 Feb 2023
SRR3126588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263027
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213220.942189377325149No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51480.22748292441937282No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG27840.12302106868367015No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG24920.11011799682460705No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC23280.10287106605444832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68500.083.275111
ATAGCGG4550.074.415961
TAGGGCA11550.072.430894
AGTAAGG13200.071.252651
AGGGTAC12050.070.985585
AGTAGGG40700.070.902212
GTACGGG5250.070.721852
ACGGGAT6000.070.498044
TAGGGTA7850.070.049014
CGTAGGG7250.069.3635942
ATAGGGC12550.069.2809453
TAAGGGA18200.068.432173
AAGGGTA15900.068.281134
GTAGGGA17350.067.9923553
AGGGAAT23100.067.954835
GGTAAGG13000.067.646171
AAGAGGG76200.067.414142
TAGGGAT15900.067.394364
ATAAGGG32200.066.557162
TATAGGG16050.066.178852
Produced by FastQC (version 0.11.5)