FastQCFastQC Report
Mon 27 Feb 2023
SRR3126587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852572
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150690.8134096812431583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27870.15043949708837226No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG19870.10725629017387718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53400.082.8471451
ATAGCGG4150.081.653611
ATAGGGC10150.077.324963
TAGGGTA6300.073.10634
AGTAGGG29100.072.868612
ATAGGGA15900.072.121043
TAGGGCA9150.071.9078454
AGTAAGG9900.069.8832551
AGGGTAC9500.069.753315
GAATAGG12750.069.3966751
TAAGGGA14700.069.376393
AGGGATG24900.068.891055
ATGCGGG8400.068.8466342
CGAAGGG18000.068.4361652
AGGGAAT18250.067.984575
AATAGGG27750.067.9419862
GTATAGG7800.067.579411
GTACGGG3900.067.5118942
TATAGGG16650.067.207792
GTAGGGA13150.067.18953
Produced by FastQC (version 0.11.5)