FastQCFastQC Report
Mon 27 Feb 2023
SRR3126586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900689
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150110.7897662374012793No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28820.15162922498104633No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG20900.10996012498625499No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC19150.10075293748740587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56600.084.993681
TAGGGCA9250.077.226514
CGTAGGG5900.074.079062
ATAGGGC10250.072.443223
ATAGGGA15700.072.440313
ATAGCGG3950.071.430151
TAAGGGA14200.070.8255543
TAGGGTA6250.070.682584
AGTAAGG10600.069.650011
AGGGATG24300.069.624295
CGAAGGG18600.069.231252
AGTAGGG30650.068.99962
TAGAGGG35500.068.310262
ATGAGGG33150.067.765542
AGGGAAT18150.067.58165
ATAGAGG13200.067.331031
ATGCGGG9250.067.065132
AGGGCAT16450.066.852025
GAATAGG12600.066.805141
ATAAGGG26850.066.687622
Produced by FastQC (version 0.11.5)