FastQCFastQC Report
Mon 27 Feb 2023
SRR3126585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125463
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190230.8950049942059684No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32130.15116706336454694No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG23800.11197560249225698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64550.082.2558751
ATAGCGG4650.080.910761
CGTAGGG7100.074.7985152
TAGGGCA10550.072.611934
TAGGGTA7900.071.98324
AGGGATG29650.071.803635
ATAGGGA18150.071.72593
AGTAGGG35550.071.2560962
AGGGTAC11400.070.9081955
AGAGGGC27150.069.240953
AAGGGTA15750.069.227764
TAGAGGG37050.069.005472
TATAGGG17650.068.964872
ATAGGGC10500.068.929373
AAGAGGG71200.068.3176962
ACGGGAT5700.067.6101464
GTAGGGA16200.067.014663
AGGGAAT20250.066.608515
GTACGGG3600.066.5795062
AATAGGG29400.066.3397142
Produced by FastQC (version 0.11.5)