FastQCFastQC Report
Mon 27 Feb 2023
SRR3126584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340720
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112510.839175965153052No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATGCTCA37830.28216182349782204No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29500.22003102810430217No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG26610.19847544602899936No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG26370.19668536308848975No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC25720.19183722179127632No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13920.10382481054955546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40300.085.2336041
CGTAGGG3450.074.959842
TAGGGCA7550.072.208024
ATAGGGC10950.072.10573
TAGACGG1900.071.818691
AGGGATG16550.071.561095
AGTAGGG23600.071.327352
GGTAAGG7950.071.024331
AGGGTAT5700.070.9084555
AGTAAGG8100.070.870881
GTAGGGC5200.070.496193
AAGGGCG4800.070.496194
ATAGGGA10550.070.384833
ACGGGAT2550.070.035444
AGACGGG8850.069.600612
ATAGCGG2650.069.248721
TAGGGTA4700.068.9962844
AGGGAAT15500.068.525335
GTAGGGT5900.067.708213
GAATAGG8550.067.691171
Produced by FastQC (version 0.11.5)