FastQCFastQC Report
Mon 27 Feb 2023
SRR3126581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090433
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242951.162199410361394No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30750.14709871112826864No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25620.12255834078394284No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC22080.1056240501369812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85350.086.589761
TAGGGCA10800.075.281084
AGGGCAT19000.074.4519965
AGTAGGG36050.072.661512
CGTAGGG7200.072.501162
AGGGATG33000.071.491315
GTAGGGC10500.071.1657943
GTAGGGA17000.070.217973
AGGGTAC10700.069.8355945
ATAGGGC14750.068.821673
TATAGGG19100.068.695022
GAGGGAT26700.068.6462554
GAGGGTA12700.068.459154
ACGGGAT5850.068.2851944
AGAGGGC31700.068.1964953
ACGGGTA2150.067.762084
CGAAGGG20550.067.7383042
AAGGGCA31550.067.180114
TAAGGGA18400.067.1740343
GGTAAGG12500.067.031731
Produced by FastQC (version 0.11.5)