FastQCFastQC Report
Mon 27 Feb 2023
SRR3126580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130243
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244291.1467705796944292No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31310.1469785371903581No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25390.11918828039805787No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC22990.10792196007685508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83100.085.5527651
CGTAGGG6750.073.094252
AGGGATG34400.072.26125
ATAGCGG4050.071.981341
AGGGAAT24600.070.4854055
TAAGGGA19800.070.248083
GTACGGG5100.070.023072
ATAGGGA18950.069.6795043
AGTAGGG32950.069.164632
ACGGGAT5400.068.7450264
TAAGAGG15100.068.5060651
AGGGCAT19600.067.84825
GAGGGAT28750.067.829434
ATGCGGG11650.067.761212
GTAGGGA17850.067.6554643
AGGGTAC9800.067.608455
GAATAGG12700.067.3830951
GGTAAGG12600.067.1715241
TAGGGCA11350.067.069824
TAGAGGG38000.066.650422
Produced by FastQC (version 0.11.5)