FastQCFastQC Report
Mon 27 Feb 2023
SRR3126579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385947
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308451.2927780876943202No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36990.15503278153286723No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28520.11953325031947482No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC25900.10855228552855534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97950.085.866271
ATAGCGG4750.078.233551
CGTAGGG7800.075.921042
AGGGATG39950.074.117225
ACGGGTA4000.071.674584
AGTAGGG40950.071.502362
TAAGCGG5850.069.955681
AGGGCAT22450.069.923875
TAGAGGG40850.069.606462
TAAGGGA23100.069.584013
GAGGGAT31850.069.355964
ATAGGGA23350.069.040283
AGAGGGC35400.068.375313
TAGGGAT21000.068.2615054
GTAGGGC10150.068.068573
ATGCGGG10250.067.86162
TAGGGCA12500.067.67964
GAATAGG14950.067.0190051
GTAGGGA23800.066.550033
GGATGGC36000.066.320457
Produced by FastQC (version 0.11.5)