FastQCFastQC Report
Mon 27 Feb 2023
SRR3126578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258885
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168870.7475812181673702No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44410.19660142061238176No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG25940.11483541658827254No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC22980.10173160652268709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62600.082.764171
TAGGGCA14400.078.3285754
ACGGGAT5250.074.300254
ACGGGTA2350.073.99554
ATAGGGC14950.072.303293
AGGGATG34850.072.14775
AGTAGGG37400.071.433852
GTAGGGC12050.070.983243
GTAGGGA18350.070.4316943
TAAGGGA19500.070.134193
ATAGAGG15050.068.788461
TAGGGAT15800.068.711014
CGTAGGG6500.068.020812
TAGGGTC7400.067.955334
CTAACGG1600.067.645241
GTTAGGG12250.067.5777742
TAGACGG2650.067.478761
AGTACGG3300.067.021381
AAGAGGG77950.066.978312
TGTAGGG17600.066.544782
Produced by FastQC (version 0.11.5)