FastQCFastQC Report
Mon 27 Feb 2023
SRR3126577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299736
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172520.7501730633429228No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44090.19171765802683438No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG28480.123840301669409No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC25370.11031701030031274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62650.081.981931
TAGGGCA13800.073.8876954
AGGGATG35400.072.473775
ATAGGGC15200.071.410133
CGTAGGG5800.071.291332
GGATAGG15150.069.232311
AGTAGGG39050.069.187692
TAAGGGA18900.068.618113
GGTAAGG13250.068.5106351
AGAGGGC33250.068.256963
GTAGGGA18850.068.052293
AAGAGGG78250.067.97422
ATAGGGA19450.067.885683
ATGCGGG12200.067.7851942
ATAGAGG15850.067.3617251
GAATAGG16450.067.1921541
ATAACGG2250.066.893521
TAGCGGG14200.066.8413242
AGTAAGG15700.066.806981
CGAAGGG21250.066.777482
Produced by FastQC (version 0.11.5)