FastQCFastQC Report
Mon 27 Feb 2023
SRR3126576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569686
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216380.8420484059141857No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50000.19457630231864906No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG29850.11616205248423349No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC26800.10429289804279589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77650.082.555621
ACGGGTA3250.078.088994
AGGGATG39750.071.176865
CGTAGGG7350.070.976582
AGTAGGG40250.069.2417762
AGAGGGC39950.068.3500443
CGAAGGG23350.068.232652
GTACGGG6150.068.0133742
GTAGGGA21350.067.360143
AAGAGGG90400.067.325682
GGTAAGG14250.066.999791
ACGGGAT6250.066.925164
GAATAGG14700.066.868431
TAGACGG3400.066.3979341
AGGGCAT22350.066.23885
TAGGGCA15050.066.203164
ATAGGGA20400.065.658983
GTAGGGC14150.065.4318543
TAGAGGG47600.065.264042
AAGGGCA42200.064.371674
Produced by FastQC (version 0.11.5)