FastQCFastQC Report
Mon 27 Feb 2023
SRR3126575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205908
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209670.9504929489353137No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33750.1529982211406822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73800.084.560381
CGTAGGG7050.077.366772
TAGGGCA11400.074.612774
AGGGATG32550.074.3525165
ATAGGGC13850.071.9325563
AGTAGGG36600.070.402012
TAGGGTA8750.070.356254
GTAGGGA18700.069.862263
GAGGGAT26300.069.507764
ATAGCGG5850.069.186661
GGTAAGG11800.068.6003341
ACGGGAT5500.068.354444
ACGGGTA2550.068.1869054
TAGGGAC9450.068.12844
CGAAGGG19950.068.114652
TGTAGGG15400.068.087862
TAGGGAT14250.067.9347154
TACGGGT1800.067.879753
AGGGAAT22950.067.777375
TATAGGG17500.067.709262
Produced by FastQC (version 0.11.5)