FastQCFastQC Report
Mon 27 Feb 2023
SRR3126574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227115
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212020.951993947326474No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31870.14309992972971758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77000.084.422211
CGTAGGG6650.079.14372
ATAGCGG4850.072.7375951
AGGGATG35300.072.685485
AGTAGGG34500.071.9175342
GGTAAGG13150.071.1814351
ACGGGAT5200.069.585234
ATAGGGC14000.068.810633
ATAGGGT10250.068.3112263
TAGGGCA12400.068.21514
ATAGGGA17050.068.077293
GTAGGGA17300.067.636783
AAGAGGG77450.067.5295262
CGAAGGG22900.067.51192
TATAGGG19650.067.199142
AATGCGG4550.067.195691
TAGGGTA7000.067.1323244
AGGGAAT24200.066.9936145
AGGGACT31000.066.3960345
AGGGCAT21100.066.368735
Produced by FastQC (version 0.11.5)