FastQCFastQC Report
Mon 27 Feb 2023
SRR3126573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505184
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264191.0545732369358898No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38100.1520846372961028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92500.084.8298951
ATAGGGT11500.073.972063
AGGGATG36400.073.726175
AGTAGGG41150.072.639732
TAGGGCA15450.072.399484
CGTAGGG7850.071.246622
TAGCGGG16100.070.936112
GAGGGAT29700.070.8938454
ATAGGGA17200.070.224983
ACGGGAT6550.069.601314
TAGAGGG43750.069.504542
AAGGGTA17750.069.372914
ATAGGGC13850.069.22573
TAGGGAC12300.069.1608664
TAGGGTA9150.068.828874
TAAGGGA16650.068.31063
AGTAAGG17000.067.7971951
ATGAGGG38200.067.5454252
GGTAAGG13100.067.512031
TATAGGG21200.067.394542
Produced by FastQC (version 0.11.5)