FastQCFastQC Report
Mon 27 Feb 2023
SRR3126572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216313
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173790.7841401462699538No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37900.1710047272203881No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28230.12737370578975082No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG28180.12714810588576614No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC26930.12150810828614911No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG25280.11406331145465463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.083.24941
CGTAGGG6050.076.162032
TAGGGCA11800.074.078634
ATAGCGG4650.073.867181
AGGGATG38000.073.586025
ATAGGGC14900.073.175163
TAGGGCG4150.071.3435064
AGTAGGG37900.070.96182
ACGGGTA2850.070.9064254
ACGGGAT6900.070.834734
GTAGGGA17650.069.4956743
AGGGAAT25450.069.247735
AGGGACT35050.068.516465
AATGCGG5300.068.3591161
CGAAGGG20950.068.002732
AGGGCAT19800.067.8832865
TAGGGAC10950.067.811754
ATAGGGA21250.067.6744163
GAATAGG14400.067.637791
GTACGGG5650.067.40692
Produced by FastQC (version 0.11.5)