FastQCFastQC Report
Mon 27 Feb 2023
SRR3126571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235785
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174760.781649398309766No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37750.16884449980655564No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG31830.14236610407530242No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC30260.13534396196414236No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28660.12818763879353337No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG26590.11892914569155799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61000.083.582371
CGTAGGG7000.081.229522
AGGGATG39500.074.001295
ATAGCGG3950.073.826081
ATAGGGC14900.072.227883
AGTAGGG36250.072.206072
AGGGCAT20000.072.136385
GTAGGGT7700.071.408653
ATAGGGA19650.071.270443
TAGGGCA12200.071.261984
TAAGGGA20450.070.32083
TATAGGG19250.069.81712
GTAGGGA18000.069.70993
ACGGGAT6000.069.708344
CGAAGGG23550.069.241292
TAGGGAC10950.069.096774
GAATAGG13350.069.054191
AAGGGCG10600.068.718214
ATAAGGG27500.068.352412
GTAGGGC11300.068.2057653
Produced by FastQC (version 0.11.5)