FastQCFastQC Report
Mon 27 Feb 2023
SRR3126570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489882
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220630.8861062492118101No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43940.1764742264894481No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC35170.14125167377409853No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG33910.1361911929962946No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33140.13309867696541441No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG28050.11265594112492078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78850.083.145561
CGTAGGG6750.075.893412
AGGGCAT22150.073.839175
TACGGGT2000.072.847233
ACGGGTA3650.072.106854
AGGGAAT31400.071.994085
ATAGCGG4900.071.985191
TAGGGAC12700.071.792544
ATAGGGC17450.071.6419753
AGGGATG41350.071.605515
ATAACGG2700.071.416431
TAGGGCA15500.070.952144
AGTAGGG42250.070.4138952
TAGGGTC7550.069.096714
GAATAGG14950.068.893921
TAAGGGA22750.068.173233
AGAGGGC35000.068.080273
AAGGGAC26400.068.004994
AGTACGG3600.067.932691
AGGGTAT13950.067.717855
Produced by FastQC (version 0.11.5)