FastQCFastQC Report
Mon 27 Feb 2023
SRR3126568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956246
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142410.7279759294076512No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27500.1405753673106552No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25020.1278980250950034No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG21590.11036444291771076Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52750.085.2531051
AGGGATG40600.075.701495
CGTAGGG6350.075.486292
ATAGCGG4500.075.265291
AGTAGGG32800.074.5025562
TAGGGCA10000.073.3123864
CGGGTAT1950.072.300185
ACGGGAT6450.072.132054
TAGCGGG12100.071.073452
GAGGGAT30950.070.606564
ATGCGGG11700.070.290052
GTAGGGC11250.070.179383
GGATGGC32400.069.767457
TAAGGGA20750.069.303643
GTAGGGA16850.068.052283
AAGGGAT41200.067.983234
AGGGCAT17400.067.521725
AAGAGGG68000.067.4500962
AGGGTAC8750.067.135885
AGGGACT29550.067.113175
Produced by FastQC (version 0.11.5)