FastQCFastQC Report
Mon 27 Feb 2023
SRR3126567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199133
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182140.8282354909866751No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31450.1430109047520091No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28470.12946010996151663No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG24270.1103616743507555Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66900.085.342371
TAGGGCA14100.079.331114
ACGGGTA2350.077.997824
ATAGCGG4150.077.060711
CGTAGGG7300.075.3249362
ACGGGAT6500.075.197894
AGGGATG46850.073.3319855
GAGGGAT38250.071.020234
AAGAGGG75000.070.809742
GGATGGC37950.069.970097
AGTAGGG36800.069.7301562
ATAGGGC14250.069.5910343
AGGGCAT19100.069.3907245
GTAGGGA18050.069.000833
GTACGGG5800.068.875812
TAGGGAC13000.068.690384
ATAACGG2150.067.810261
AAGGGAC24200.067.779094
TAGCGGG11800.067.708432
AGAGGGC30950.067.271133
Produced by FastQC (version 0.11.5)