FastQCFastQC Report
Mon 27 Feb 2023
SRR3126566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732115
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127690.7371912373023731No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28210.16286447493382367No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG24950.14404355369014182Illumina PCR Primer Index 5 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT21720.12539583110821165No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC20660.1192761450596525Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG19830.11448431541785621Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46250.081.0000151
CGTAGGG6000.079.150262
GTAGGGA11750.073.9950943
AGTAGGG26750.072.771072
ATAGGGA14450.070.901873
GTACGGG4250.070.806522
TAGGGAT10350.070.38184
TAGGGCA7450.070.02224
TATAGGG12900.069.2541962
CCGTACA10400.068.2358553
TAAGGGA14900.067.498883
ACGGGAT3850.067.1384054
ATGAGGG27450.066.97562
TAGCGGG8850.066.9436652
ATAGGGC9550.066.435393
AGGGATG21400.066.32275
AAGAGGG55850.065.920552
GTAGGGC7850.065.85553
GGTAAGG11600.065.726541
GAGGGAT22750.065.485764
Produced by FastQC (version 0.11.5)