FastQCFastQC Report
Mon 27 Feb 2023
SRR3126565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754506
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.7371875616270335No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27990.15953208481475697No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG26900.15331950987913406Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC22630.12898217503958379Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG22240.12675932712683796Illumina PCR Primer Index 5 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGCATGATT19490.11108539953696367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCA6150.082.5207754
CGTTTTT46600.081.251851
CGTAGGG6500.079.5232242
ATAGGGC9550.074.791953
AGTAGGG27250.072.598942
TAGGGAT10400.072.2938464
GTATAGG6250.072.246121
TATAGGG13350.070.398492
GATTAGG5350.070.333051
GTAGGGA12950.070.032913
AGGGCAT14250.069.909425
AGTAAGG11150.069.603711
ATAGGGA14300.067.693323
ATAGAGG13000.066.934751
AGGGAAT16100.065.962515
AGGGATG20200.065.601295
GATAGGG22350.064.9674152
GAATAGG9950.064.762081
TAGCGGG9450.064.64372
TAGAGGG33250.064.303472
Produced by FastQC (version 0.11.5)