FastQCFastQC Report
Mon 27 Feb 2023
SRR3126564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963529
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164230.836402212546899No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31440.16011986581303356No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG29200.1487118346609599Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG24920.12691434656681924Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC24730.12594670106731298Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56350.081.887891
TAGGGCA8750.073.5854644
CGTAGGG6650.072.087182
ATAGGGC11550.071.209113
AGTAGGG31750.070.7560652
ATAGGGA17650.070.297323
GTAGGGA14750.070.098733
AAGGGGC47550.069.780434
AGTAAGG12300.069.600261
ATAGAGG13400.069.5032351
TAAACGG1900.069.318481
TATAGGG15600.068.990682
TAGGGAC8500.067.455974
TAGAGGG34850.067.294142
ATAGCGG2950.066.9687041
GGTAAGG12450.065.739211
GATAGGG27750.065.204452
ATGAGGG31850.065.074172
AGAGGGC28950.064.774473
AAGAGGG62750.064.561422
Produced by FastQC (version 0.11.5)