FastQCFastQC Report
Mon 27 Feb 2023
SRR3126563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023907
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160790.7944535000867136No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42020.2076182354228727No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34940.17263639090136057No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT31970.15796180358089576No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG30210.14926575183543514No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC28810.14234843794700053No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG23280.11502504808768388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57400.084.0401461
TAGGGCA10200.076.944774
AGGGATG37450.076.549235
AGAGGGC26800.072.423263
ACGGGTA2100.071.613274
ACGGGAT5800.071.304594
GGTAAGG13400.071.296141
AGTAGGG34950.069.817692
AGGGCAT16050.069.689095
CGTAGGG5950.069.5361942
ATAGCGG3250.069.507671
GGATGGC31750.069.125137
TAGCGGG13150.069.00442
ATAGGGA17550.068.8206563
AAGGGAT37950.068.729634
CGAAGGG21250.068.366732
TAAGAGG13500.068.327751
GTAGGGA18050.068.2160953
TAAGGGA19000.068.020823
TGTAGGG12750.067.850472
Produced by FastQC (version 0.11.5)