FastQCFastQC Report
Mon 27 Feb 2023
SRR3126562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048996
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159770.7797477398686967No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42440.20712583138278456No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34460.16817992812089433No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG33200.16203057497428008No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC32790.16002959498212782No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT28760.14036142579097274No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG26570.12967326436947654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55500.084.156751
AGGGATG38150.080.56475
ACGGGAT6100.073.961044
AGTAGGG34300.073.4327552
TAGGGCA9550.073.3237154
TAACGGG6500.073.017532
AGAGGGC27700.072.614873
GAATAGG13700.071.069371
GTAGGGT7850.070.6437763
ATAGGGA18250.070.558493
AGGGAAT23850.070.149275
TAGGGCG2550.070.033364
TAAGGGA16450.069.994153
GTAGGGC9700.069.767363
AGGGCAT16800.069.654895
ATAGGGC13500.069.275693
TGTAGGG14500.068.056632
GGATGGC30800.068.054417
AAGGGAT39400.066.9157264
CTAGCGG2750.066.7059561
Produced by FastQC (version 0.11.5)