FastQCFastQC Report
Mon 27 Feb 2023
SRR3126560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061231
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190610.9247386634491719No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27710.13443422886614845No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27420.1330273026167373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67900.085.9301451
CGTAGGG5900.081.285852
ACGGGTA2800.077.2087254
TAGGGTA9400.076.994454
TAGCGGG12650.075.823962
GTAGGGT9950.075.572453
ATAGCGG6250.074.5330351
TAGGGCA13250.073.421134
AGGGATG32600.072.657345
AGTAGGG34500.072.230992
AGGGTAC11700.070.294085
ACTAGGG9250.070.14622
ATAGGGC15750.069.525153
AAGGGTA17450.068.946334
CGGGTAT2800.068.8164755
GTAGGGC12100.068.7471163
AATGCGG5450.068.206261
TAGGGTC7400.067.954574
AGATAGG11900.067.614961
AGAGGGC32050.067.598883
Produced by FastQC (version 0.11.5)