FastQCFastQC Report
Mon 27 Feb 2023
SRR3126559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104629
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192410.914222886789073No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28080.1334201894965811No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25800.12258692624685871No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG22860.10861771837221666RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64250.083.778851
ACGGGTA2050.077.939814
CGTAGGG5150.075.732892
TAGGGCG3450.073.554454
AGTAGGG37400.073.501792
TAGGGCA13950.073.100414
AGGGTAC12300.072.973065
ATAGCGG6000.072.9311751
ATAGGGC15050.072.128983
AGGGATG33450.070.805785
AGGGACT24800.070.679175
GTAGGGA15150.070.101953
ATAGGGA20050.070.079513
AGGGCAT18900.069.868085
CGAAGGG20550.069.743172
GAATAGG13800.069.555671
ACTAGGG9400.069.4865342
AAGGGTA16650.068.86684
GGTAAGG11400.068.5148541
TAAGGGA15700.068.244793
Produced by FastQC (version 0.11.5)